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  • AutorenbildJuergen Mairhofer

Research study on phage-inspired growth decoupled recombinant protein production in Escherichia coli

Vienna, Austria: – Scientists from recombinant proteins specialist CDMO enGenes Biotech GmbH (enGenes) have collaborated with researchers from Vienna’s University of Natural Resources and Life Sciences (BOKU) on an important study of bacteriophage Inspired Growth-Decoupled Recombinant Protein Production in Escherichia coli.

The research study was authored by four enGenes scientists, Patrick Stargardt, Lukas Feuchtenhofer, and Juergen Mairhofer in partnership with Monika Cserjan-Puschmann and Prof. Gerald Striedner from BOKU.

Decoupling synthesis from cell growth

The article, accepted for publication in the American Chemical Society (ACS) Synthetic Biology journal, presents a novel approach for improved recombinant protein production (RPP) using E. coli by decoupling recombinant protein synthesis from cell growth and outlines the capabilities of the enGenes-eXpress technology within a peer-reviewed format.

The authors show that cell division and host mRNA transcription can be successfully inhibited by co-expression of a bacteriophage-derived E. coli RNA polymerase (RNAP) inhibitor peptide and that genes over-transcribed by the orthogonal T7 RNAP can finally account for more than 55 per cent of cell dry mass (CDM).


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