enGenes provides a high-quality protein expression platform for cost-effective biomolecule manufacture
Products-ServicesEnGenes Biotech GmbH
April 25th 2023
enGenes Biotech provides high-quality protein expression and process development services to enable cost-effective manufacturing of biomolecules, including recombinant protein production, secretory production of proteins and plasmids DNA production. enGenes can provide milligrams and even grams quantities of proteins and various plasmid DNA for research purposes and also specializes in upstream and downstream processes development for the large-scale production of research-grade plasmids DNA.
T7 expression
enGenes is an expert in the use of the T7 expression system to clone recombinant DNA using strains of Escherichia coli (E. coli) as an optimal system for expressing recombinant proteins. Expression of recombinant proteins under the control of the T7 promoter is up to eight times faster than basic E. coliexpression under the control of RNA polymerase.
In the T7 expression system, an expression vector such as pET is adapted to bring together a T7 promoter with a gene of interest that the promoter will control during downstream processing (DSP). The combined expression vector is transformed into an E. coli strain. The E. coli cell’s chromosomal gene can be expressed to produce T7 RNA polymerase.
Transcription is initiated when an inducer binds to the lac repressor to prevent it from inhibiting the E. coli expression of the T7 gene. T7 RNA polymerase binds to the T7 promoter on the expression vector to transcribe to its downstream gene of interest. To stimulate production and fermentation processes, the inducer reagent IPTG can be added to prevent the lac repressor from inhibiting gene expression. enGenes Biotech has extensive know-how and proprietary technologies to avoid ‘leaky expression’ and to prevent plasmid instability.
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